Briefly, 50 µl-samples were incubated with equal volumes of Griess reagent (1% sulphanilamide, 0. (Shiga, Japan) and kept for 30 min in the dark. Analysis of ALA by Ehrlich’s Reagent Assay. The cell survival. Each SpectraMax® 340 PC 384 Microplate Reader has an optical system built around a monochromator. 2. SpectraMax 190 reads 96-well and strip-well plates. Donor filter: 616/10 nm. Title: SPECTRAmax 340 Microplate Reader manual Author: Research Facilities Keywords: spectramax, 340, microplate reader, manual, Created Date: 12/13/2005 9:07:53 AMSpectraMax Multi-Mode Microplate Readers. In this study we used tetanus toxoid (TT) as a well-defined antigen to. WST regents of 10% were added to each well, and absorbance was determined at 450 nm using an ELISA reader (SpectraMax® 340, Molecular Device Co. 6. ” SpectraMax® Quant™ dsDNA Assay Kits The SpectraMax® Quant™ dsDNA Assay Kits are designed for fluorescence-based dsDNA quantitation across a broad range of concentrations. NO release assayThe SpectraMax® M3 Multi-Mode Microplate Reader dual monochromatic optics allows the widest range of applications to be utilized for bioresearch applications all without the need to change filters, and provides three modes of detection in one platform. When readin g optical density at wavelengths below 340 nm, special UV-transparent, disposable or quartz microplates and cuvettes that allow transmission of the far UV spectra must be used. Scanning was done at 600 nm, using a Molecular Devices SpectraMax 340 plate reader. Absorbance was then measured at 540 nm (isosbestic point of cytochrome c) and 550 nm (SpectraMax 340; Molecular Devices, Sunnyvale, CA, USA). SPECTRAmax 340PC Microplate Spectrophotometer Operator’s Manual 1-3 Introduction General Overview The SPECTRAmax ® 340PC incorporates a holographic grating monochromator which allows you to specify a precise wavelength, from 340 nm to 850 nm, for the absorbance maximum of your sample. The absorbance was measured at 340 nm for 2 min at a 15 s time interval. The extinction coefficient at 280 nm used for determining the concentration of Rsp3690 was calculated to be 28,795 M –1 cm –1. Awareness Technology Inc. With SOFTmax PRO, the contents of the wells in a microplate can be mixed automatically by shaking before each read cycle, making it possible to. , Oxon, UK). 00. Driessen*,MaaikeA. After washing, the dye was eluted with 3% HCl, and the absorbance measured with a SpectraMax 340 microtiter plates reader at 665 nm. The spectrophotometers used were SpectraMax 340 (for GSH, GCS, and HNE-His assays) and SpectraMax M3 (for GGT, GPx, and GST assays), both from Molecular Devices, Sunnyvale, CA. Optical density was read at 405 nm after 30 min using a Molecular Devices SpectraMax 340 spectrophotometer. Revvity. SpectraMax M2, while top and bottom reads are possible on the SpectraMax M2 e. An absorbance was read at 490/630 nm using a spectraMAX 340 microtiter plate reader. DNA/RNA concentration is commonly estimated by dividing the A260 value by the 1-cm absorptivity value (or multiplying by its reciprocal). 5 mm (bottom read) Beam diverges to focal spot near bottom of well. 1 mL of culture media with 10% FBS. 8 pin mini DIN (Spectramax 340) to 9D (PC) serial data transfer cable. The ratio of red to green fluorescence intensity was calculated. Absorbance was measured at 450 nm by using a SpectraMax 340 microplate spectrophotometer (Molecular Device). Supplier: Molecular Devices. Water – distilled water or equivalent. Description 1. Cell permeabilization. Laser Scanning Confocal Microscopy The fluorescent signals were obtained using a Zeiss LSM 510 laser scanning confocal microscope. Awareness Technology, Inc. 3: The cuvette chamber. (7. Isolation of RNA and RT-PCR. The OD for each serum is adjusted by subtracting either the OD for spontaneous mediator release or antigen-specific mediator release. Cell viability was determined using the blue formazan assay in which colorless 3-(4,5-dimethyl-thiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) is metabolized to a blue product by mitochondrial dehydrogenases. SpectraMax i3x 和 SpectraMax iD5 微孔板读板机上使用 SoftMax Pro 软件中的预设方法进行测量(有关仪器特定设 置，请参见表 2 和表 3 )。在 SpectraMax i3x 和 SpectraMax iD5 读板机上进行了微孔板优化和读取高度调整，以确保最佳 的检测灵敏度和动态范围。 数据分析SpectraMax® Absorbance Microplate Readers can run both cuvette-based and microplate reader applications on the same instrument. These models can no longer be repaired. Absorbance was measured at 490/650 nm by using a SpectraMax 340 microplate spectrophotometer® (Molecular Devices, Sunnyvale, CA, U. The SpectraMax™ GEMINI EM Dual-Scanning Microplate Spectrofluorome-ter can perform a variety of fluorescent applications as well as some lumines-cent assays. The optical system of the SpectraMax 340PC384 Microplate Reader is built around a monochromator, which allows for selection of up to six wavelengths at a time for absorbance detection in the visible wavelength range (340 nm - 850 nm). The SpectraMax® M Series Multi-Mode Microplate Readers are modular and upgradeable with a wide range of high performance capabilities ideal for life science research and drug discovery screening SoftMax® Pro 7. SpectraMax Multi-Mode Microplate Readers. All ELISA plates were analyzed using a SpectraMax 340 PC microplate reader (Molecular Devices). Assay buffer was 50 m M Hepes, pH 7. The optical density (OD) at the wavelength of 450 nm was measured with a spectrometer (SpectraMax 340, Sunnyvale, CA, USA). The cell growth was determined by measuring the optical density at 600 nm using a spectrophotometer (SpectraMax 340, Molecular Devices, USA). The inhibition of ALDH activity by compounds and the IC50 curves were determined by measuring the formation of NAD(P)H spectrophotometrically at 340 nm (molar extinction coefficient of 6200 M −1 cm −1) on a Beckman DU-640 as well as a Spectramax 340 PC spectrophotometer (GMI, Ramsey, NJ, USA) using a purified. Products : SpectraMax Paradigm reader, SpectraMax Plus 384 reader, SpectraMax 250 (Obsolete), SpectraMax L reader, SpectraMax 340PC 384 (Obsolete), Gemini EM reader, Gemini XPS reader, SpectraMax 340 (Obsolete). Molecular Devices Corporation 1311. Features showcased by this model include temperature control, a robotics-compatible interface and Data Analysis Software. 4) was followed according to Vanacker et al. Near bottom. Molecular Devices SpectraMax 340 PC 384 Microplate Reader. 305 reference level saturation. Plates were read at a wavelength of 405 nm with a reference wavelength of 620 nm (Spectramax 340 PC). 5 ng/ml; estradiol: 10 pg/ml. Top and bottom reads are available for fluorescence, ti me-resolved fluorescence and luminescence detection. 2. Confocal laser scanning microscopy (CLSM) Biofilms were formed on glass-bottomed Petri dishes (CELLview™; Greiner Bio-One GmbH, Frickenhausen, Germany) in the presence or absence (control biofilms) of the BIC 50 of antifungals as described above. #N207) LAL Reagent Water (Lonza cat. Call 1-800-745-2710. 2. Call 1-800-745-2710. Effect of hypoxia on natriuretic peptides and VEGF production in AC16 cells. The values were corrected for nonspecific background by subtracting the OD 650. 0112-0126B 8 Chapter1:Introduction TheSpectraMax®340PC384,SpectraMax®190,andVersaMax™microplate. TGFα release was calculated as described in the Results. For cell permeabilization, 1 ml of Z buffer (60 mM Na 2 HPO 4 z 7H 2 O, 40 mM NaH 2 PO 4 z H 2 O, 10 mM KCl. 4- to 5-weeks old female BALB/c mice were purchased from Charles River Laboratories (QC, Canada). StakMax Microplate Handling Stacker. One plate carrier adapter is provided with the instrument. Includes: Computer Loaded with Softmax Pro 5. The contents of the. After treatment, total cellular RNA was extracted with a cold RNA extraction solution (Ultraspec RNA;Call 1-800-745-2710. 0112-0126B 8 Chapter1:Introduction TheSpectraMax®340PC384,SpectraMax®190,andVersaMax™microplate. Briefly, hPDLSCs were cultured in osteogenic/odontogenic induction medium for 14. 1: SpectraMax Plus 384 components. Molecular Devices SpectraMAX 340 Microplate Reader. The SpectraMax ABS/ABS Plus Microplate Readers provide fast absorbance detection without the use of filters with monochromator-based wavelength selection for visible and. Subsequently, the oxidative burst was initiated by the addition of PMA (final concentration 100 nM) and the cells incubated for 120 min at 37°C. A 0. Absorbance plate reader, temperature control, robotics-compatible, 384 well plates, 340-850 nm. The reaction mixture consists of 100 mM potassium phosphate buffer (pH 7. The reaction was then stopped by adding 50 µL of 1 M sulfuric acid to each well. The levels of rheumatoid factor (RF) antibodies were measured at the Clinical Immunology Laboratory of the Sahlgrenska University Hospital or Leiden Clinical Immunology Laboratory. SpectraMax Multi-Mode Microplate Readers. With SOFTmax PRO, the contents of the wells in a microplate can be mixed the industry-leading microplate reader software for the SpectraMax Plus 384 system, allows complete customization of data collection and analysis. Created Date: 20051213090753Zmeasured using a SpectraMax 340 multiwell plate. Optical density was measured using a SpectraMAX 340 (Molecular Devices, Sunnyvale, CA). Molecular Devices SpectraMax 384 Plus Microplate Reader Specifications. The cytotoxicity of the CDDP loaded CHAMs were done by treating the FaDu cells with DMEM supplemented with 0. The values were compared against a standard curve that was generated using known concentrations of IL6 to calculate concentration in the. Microplate Spectrophotometer Operator’s Manual. The SpectraMax® absorbance spectrophotometers and plate readers provide the versatility and convenience for a wide range of assays such as ELISAs, nucleic acid and protein quantitation, and microbial growth. The Molecular Devices SpectraMax 340PC384 Microplate Reader can run both standard spectrophotometer and microplate reader applications on the same instrument. 340/70 nm. Thin layer chromatography (TLC) was. 2. Created Date: 20051213090753Z measured using a SpectraMax 340 multiwell plate. Molecular Devices. Absorbance was then measured at 540 nm (isosbestic point of cytochrome c) and 550 nm (SpectraMax 340; Molecular Devices, Sunnyvale, CA, USA). A. Furthermore, the stain was extracted using 10% acetic acid, and its absorbance was measured at 595 nm in a microtiter plate reader (SpectraMax 340; Molecular Devices). reader (Molecular Devi ces). After then, 10% of ammonium hydroxide solution was added to neutralize the aqueous solution of the ARS extracts, and the absorbance values were measured using a SpectraMax® 340 plate reader at 405 nm. The plates were incubated for 4 additional hours at 37°C. , Fremont, CA, USA). Application note showing how SpectraMax® i3x and SpectraMax®iD5 Multi-Mode Microplate Readers are used to performGq-coupled receptor assays using HTRF technology. 314 low lamp level warning. 4) was followed according to Vanacker et al. Absorbance was read at 405 nm on a microplate reader (SPECTRAmax 340 PC). Molecular Devices VersaMax Absorbance Microplate Reader, 340-850 nm, Molecular Devices (CAT#: STEM-SPO-0005-YJL) Labsystems Fluoroskan II Fluorescent Microplate Reader, 320 – 700 nm. 10. Page 2 1) All labor charges to repair the product for a period of one (1) year from the original date of. , Sunnyvale, CA). Setup recommendations for SpectraMax. 5. All assays were performed in triplicate, and the absorbance at 405 nm was measured using a SpectraMax 340 multiwell plate reader (Molecular Devices). SpectraMax Injector Cartridge with SmartInject™ Technology. The SpectraMax® ABS Plus Microplate Reader can run both cuvette-based and microplate reader applications on the same instrument. Molecular Device M2e. Labsystems Luminoskan RT Microplate Reader. LAL Reagent Grade ™ Multi-well Plates (Lonza cat. Glutathione reductase (GR) activity was measured, according to Smith et al. Availablility: By Availability Molecular Devices SpectraMAX 340 Microplate Reader. Media in plates were withdrawn by pipetting and 200 μl of DMSO was added to each well to solubilize the formazan crystals. PerkinElmer offers a variety of Microplates For Absorbance and Colorimetric Assays. 5. These robust readers have been placed in labs from Antarctica to the. spectramax i3 multi-mode platform, spectramax i3, spectramax i3 system, scanlater western blot system, spectramax minimax imaging cytometer, softmax pro software, metamorph software, spectramax i3 platform. When readin g optical density at wavelengths below 340 nm, special UV-transparent, disposable or quartz microplates and cuvettes that allow transmission of the far UV spectra must be used. Not all manufacturers' microplates are the same with regard to design, materials, orIn a typical 100-μL assay with mixtures containing 50 mM MOPS, pH 6. Six wells were assayed for each set of conditions and stadard deviations. ). automated SpectraMAX 340 (Molecular Devices, Sunnyvale, CA, USA) microtiter plate reader. When readin g optical density at wavelengths below 340 nm, special UV-transparent, disposable or quartz microplates and cuvettes that allow transmission of the far UV spectra must be used. After 72 h, 20 μl of 5 mg/ml MTT solution was added to each well and plates were incubated for 3 h at 37°C. 3T3 cells were cultured in DMEM supplemented with 10% FCS and 1% antibiotics (10,000 units/ml penicillin and 10,000 μg/ml streptomycin in 0. 1 mL of acetylacetone. Near bottom. Continuous kinetic monitoring of enzyme activity was performed on SPECTRAmax 340 or Flexstation 2-384 microplate reader (Molecular Devices, Sunnyvale, CA, USA) and controlled by the Softmax software. LDH release was measured with a commercial kit following the manufacturer’s instructions (LDH Cytotoxicity Detection Kit, TaKaRa). 314 low lamp level warning. 0 2. For 40 years, we have partnered with scientists to help them achieve landmark discoveries. The drawer will open and it may close, if is doesn't, close it using the DRAWER button found on the Spectramax control panel (pictured in purple on. 30. The Molecular Devices SpectraMax 340PC384 Microplate Reader can run both standard spectrophotometer and microplate reader applications on the same instrument. When readin g optical density at wavelengths below 340 nm, special UV-transparent, disposable or quartz microplates and cuvettes that allow transmission of the far UV spectra must be used. PGD2 concentrations in samples from treated cell cultures were determined using the linear range of a standard curve and are presented as percentages of the PGD2 levels measured in the solvent controls. SPECTRAmax® 190. Manager: Yinghua Chen, PhD Contact Email: yinghua. SpectraMax and VersaMax Plate Readers Operator’s Manual — 0112-0126 Rev A SpectraMax Plus 384 SpectraMax 190 SpectraMax 340PC 384 VersaMax. TDP-43 stress granular cells were treated with 70% ice-cold. Time-resolved fluorescence was measured on the SpectraMax i3x and SpectraMax iD5 readers using preconfigured protocols in SoftMax® Pro Software (see Tables 2 and 3 for instrument-specific settings). The SpectraMax ABS Plus, can accommodate standard 96-well plates and 384-well plates. edu Contact Phone: 216-368-8962. Features demonstrated by the model include temperature control, a robot-compatible interface and data analysis software. Quick View. Availablility: By Availability Molecular Devices SpectraMAX 340 Microplate Reader. The SpectraTest validation package provides a NIST-traceable solution for validating optical performance of the SpectraMax Plus 384 reader and does it automatically. The SpectraMax 340PC 384 and SpectraMax Plus 384 read both 96-and 384-well microplates. 340 nm / 80 nm. The SpectraMax® Plus 384 Microplate Reader from Molecular Devices can run both standard spectrophotometer and microplate reader applications on the same instrument. Our absorbance plate readers feature our PathCheck Sensor technology and. Lactate Dehydrogenase (LDH) Release. Title: SpectraMax i Series Multi-Mode Microplate Readers | Molecular Devices Author: Molecular Devices Subject: SpectraMax i Series offer the ideal solution for any application whether you are looking for an intuitive, easy-to-use reader, multi-user lab, or an upgradeable system that can adapt to support your lab s new projects. SpectraMax 340PC 384 and SpectraMax Plus 384 read both 96-and 384-well microplates. Assay setup for a 384-well low-volume plate. 2. Please see photos. ir Neuroprotection of a sesamin derivative, 1, 2-bis [(3-methoxy- phenyl) methyl] ethane-1, 2-dicaroxylic acid (MMEDA) againstEach SpectraMax® 340 PC 384 Microplate Reader has an optical system built around a monochromator. One plate carrier adapter is provided with the instrument. TheSpectraMax®340PC384,SpectraMax®190,andVersaMax™microplate spectrophotometersproviderapidandsensitivemeasurementsofavarietyofanalytes. Top and bottom reads are available for fluorescence, ti me-resolved fluorescence and luminescence detection. SpectraMax ® Plus 384 optics. The SpectraMax 340PC384 Microplate reader supports various absorbance assay categories, such as protein quantitation, endotoxin detection, microbial growth, ELISAs. Not all manufacturers' microplates are the same with regard to design, materials, or76358-624. Molecular Devices SpectraMax 340 PC 384 Microplate Reader. The contents of the wells in a microplate can be mixed automatically by shaking before each read cycle, which makes it possible to perform kinetic analysis of solid- pathways and protein activation and expression in one system. The contents of the wells in a microplate can be mixed automatically by shaking before each read cycle, which makes it possible to perform kinetic analysis of solid- Absorbance at 405 nm (Abs 405) was obtained for both plates before (background) and after 1-h incubation at 37°C using a microplate reader (SpectraMax 340 PC384, Molecular Devices). Finally, after another 45 min of decolorization, 100 µL of the solution was transferred to a new well and the amount of crystal-violet stain in the decolorizing solution was measured with a microtiter plate reader (SpectraMAX 340 Adjustable Microplate Reader; Molecular Devices Ltd. Molecular Devices SpectraMax M5e Microplate Reader. With a wide range of on-board features and capabilities, thermoax is ideally suited for use in modern laboratories involved in many applications. About The SpectraMax GEMINI EM is controlled by an external computer running SoftMax ® Pro software which provides integrated instrument control, data display, and statistical data analysis. , Sunnyvale, CA). 3. , Sunnyvale, CA, USA) at 595 nm. ThermoMax performs automatic mixing before and between readings to ensure reliable and precise. #25-340) Pyrogen-free Dilution Tubes (Lonza cat. On 1, 7, 14, and 21 days, the cells were washed with DPBS twice and incubated with CCK-8 solution for 2 h in the dark at 37 °C. Spectral Range. Molecular Devices SpectraMax 340 PC 384 Microplate Reader. Read one sample or up to 384. Wavelength(s) 340 to 850 nm; Add to Compare List. The absorbance values were measured at 450 nm using a SpectraMax® 340 plate reader (Molecular Devices, Sunnyvale, CA, USA). 5. TheSpectraMax®Plus384addstheabilitytoread SpectraMax Spectrophotometers for UV-Vis Absorbance Detection. Details. When you do reads at wavelengths below 340 nm, you must use special UV-transparent, disposable or quartz plates to allow transmission of the deep UV spectra. Microplate Readers. Quick View. The SpectraMax® 190 Absorbance Microplate Reader is the ideal equipment for many life science applications including DNA analysis. Percent radical scavenging activity was determined by comparison with a DMSO containing control (shown in Table 3). 97), and each plate contained at least six positive and six negative controls. When reading optical density at wavelengths below 340 nm, special UVtransparent, disposable or quartz microplates and cuvettes that allow transmission of the far UV spectra must be used. HTRF-compatible SpectraMax Multi-Mode Readers. Perkin Elmer Wallac Victor2 1420 Multilabel Counter $1,500. Amino Acid Analysis. As discussed below, dilution of the culture was required because cell density is not proportional to A 600 when the values are above ;0. Immunofluorescence and Aggregation Analysis. Molecular Devices SpectraMax Gemini XS Microplate Reader. , Sunnyvale, CA) at 490 nm. When you do a read at wavelengths below 340 nm, you must use special UV-transparent, disposable or quartz plates to allow transmission of the deep UV spectra. 306 plate air cal fail,low light. 0112-0115G October2018 SpectraMax®M3 SpectraMax®M4 SpectraMax®M5 SpectraMax®M5e Multi-ModeMicroplateReaders UserGuideHigh-throughput assays for promiscuous inhibitors Brian Y Feng1,3, Anang Shelat1,3, Thompson N Doman2,RKipGuy1 & Brian K Shoichet1 High-throughput screening (HTS) searches large libraries of chemical compounds. SoftMax Pro. The SpectraMax GEMINI EM cannot be operated without the computer and SoftMax Pro software. 1% N-1-naphtylethylenediamine dihydrochloride, 2. Spectrophotometer (SpectraMax 340). At that point halfway up the maximum transmittance value, the total width (nm) of the transmitted band is. 2. TGFα release was calculated as described in the Results. Plate reader (SpectraMax 340 PC plate reader or comparable plate reader). Top and bottom reads are available for fluorescence, time-resolved fluorescence and luminescence detection. Molecular Devices Spectramax 340PC instructions. Cells were scraped from the plate and single cell suspensions were made by passing cells through 28G1/2 insulin syringes. No power cord or accessories included. Optical density was determined at 490 nm (background values measured at 690 nm were subtracted) using a SpectraMax 340 microplate reader (Molecular Devices, Sunnyvale, CA, USA). The Analyst®, FlexStation® and SpectraMax® M5/M5 e from Molecular Devices have received the LanthaScreen® Certified designation from Life Technologies which ensures that these readers are validated to strict standards in instrument setup and assay performance. Stat Fax 4700 Microstrip Reader. When reading optical density at wavelengths below 340 nm, special UVtransparent, disposable or quartz microplates and cuvettes that allow transmission of the far UV spectra must be used. Filipin StainingSpectraMax® 340PC384 microplate reader provides everything needed to measure absorbance in the visible range, including temperature control, a robotics-compatible interface and SoftMax® Pro data analysis software. well, and strip-well microplates. SpectraMax 340 (Serial number starting with M) SpectraMax 340PC (Serial number starting with L) SpectraMax Plus (Serial number starting with P) Tmax Also refer. Immunofluorescence and Aggregation Analysis. Briefly, hPDLSCs were cultured in osteogenic/odontogenic induction medium for 14. 5. Examples: “Press carefully to engage,” “Do not press down. SpectraMax Microplate Readers. . Although it has been proven to be effective in various neurologic disorders, the mechanism of the treatment of traumatic brain injury (TBI) is not fully understood. The first time you replace an OEM lamp you must use the N-34-MLA. Statistical AnalysisConsult Molecular Devices's entire SpectraMax i3x catalogue on MedicalExpo. 4. , San Jose, CA) using Softmax Pro 7 software. Irene Tieleman, Ido PenandSimon VerhulstSubsequently, the oxidative burst was initiated by the addition of PMA (final concentration 100 nM) and the cells incubated for 120 min at 37°C. A standard curve was constructed by sequential dilution of a TNFα standard from 2000-15 pg/mL. or microplate reader (e. 94 g calcium chloride dihydrate (both. #25-340) Pyrogen-free Dilution Tubes (Lonza cat. CSF-1, IL-34 and calprotectin levels were analysed by ELISA according to instructions provided by the manufacturer (R&D Systems, Minneapolis, Minnesota, USA). Although all samples initially were analyzed at the same volume, the volume of sample. Molecular Devices SpectraMax 340 PC 384 Microplate Reader. Read one sample or up to 384 in a single plate read using any standard cuvette, or 96- or 384-well microplate. 0, MSD) or Spectramax 340 (Molecular Devices). Both readers feature an 8-channel read head for fast reads and. SpectraMax i3x reader measures absorbance, fluorescence, & luminescence with user-upgradeable application including western blot imaging, AlphaScreen, fast kinetics along with injectors plus additional detection modes. Anti-proteolytic activity. 5 stars - 1602 reviewsThis assay typically develops in 1. All variables were tested in three independent cultures for each experiment, which was repeated twice (n = 6). S. Apoptosis was also measured by terminal deoxynucleotidyl transferase-mediated dUTP-biotin nick end-labeling (TUNEL) assay using the TACS Blue label kit (4811-30-K, R&D Systems). Stat Fax 4700 Microstrip Reader. 30. Lactate Dehydrogenase (LDH) Release. SpectraMax Multi-Mode Microplate Readers. Binding affinity for denosumab. Each experiment was performed in duplicate. The SpectraMax® QuickDrop™ Micro-Volume Spectrophotometer . 0 Micromoles per Joule, ensuring the highest light output using the least amount of electricity. The plates were incubated for 4 additional hours at 37°C. Absorbance at 490 nm was determined using a SPECTRAmax 340 (Molecular Devices Corporation). 0. Molecular Devices SpectraMax M5e Microplate Reader $26,500. The absorbance was measured at 405 nm on a SpectraMax 340 (Molecular Devices). density was measured at 570 nm (SpectraMax 340, Molecular De-vices, Sunnyvale, CA). application or budget. After adding sample, the mixture was incubated for 30 min at 37°C, with one unit of enzymatic activity being defined as an increase in absorbance of 0. Donor: 616/10 nm. MCE has not independently confirmed the accuracy of these methods. 1. Cross sections of mouse skin was prepared and stained as previously described . Top and bottom reads are available for fluorescence, time-resolved fluorescence and luminescence detection. Related Products: SpectraMax 340 Absorbance Microplate Reader, Molecular Devices SpectraMax 384 Plus UV/Vis Microplate Reader, Molecular DevicesGETTING THE MOST FROM YOUR MOLECULAR DEVICES MICROPLATE SYSTEMS applications guide to microplate systems applications guide to microplate systems450 nm using a SpectraMax 340 microplate reader (Molecular Devices, Sunnyvale, CA, USA). This tunable visible absorbance reader is for 96- and 384-well microplates and acquires data in endpoint, kinetic, and spectral scan modes using wavelengths from 340-850 nm, tunable in 1. Absorbance values were measured using a plate reader (Molecular Devices; SpectraMax 340) at λ = 405 nm. The manufacturer of the SpectraMax 340PC Microplate Spectrophotometer is Molecular Devices. Fully tested with Warranty. Since the formation of NADPH is stoichiometrically related to the oxidation of glucose-6-phosphate, measurements for glucose + fructose can be determined by the absorbance of NADPH at 340 nm. Products : SpectraMax Plus 384 reader,. Product Keyword. SpectraMax® Microplate Readers and SoftMax® Pro Software. Absorbance measurements were recorded using a SpectraMax 340 (Molecular Devices) at 570nm on the indicated days (Cell Proliferation Kit I; Roche). measurements were performed on a SPECTRAmax-340 plate reader (Molecular Devices Inc. LDH release was measured with a commercial kit following the manufacturer’s instructions (LDH Cytotoxicity Detection Kit, TaKaRa). 024). Molecular Devices SpectraMax M5e Microplate Reader. This Molecular Devices SPECTRAmax 340PC incorporates a holographic grating. 303 unable to cal dark current. Not all manufacturers' microplates are the same with regard to design, materials, orAbsorbance at 405 nm (Abs 405) was obtained for both plates before (background) and after 1-h incubation at 37°C using a microplate reader (SpectraMax 340 PC384, Molecular Devices). The absorbance at 405 nm was determined using a microplate reader (spectraMAX 340). This Molecular Devices SPECTRAmax 340PC incorporates a holographic grating monochromator which allows you to specify a precise wavelength, from 340 nm to 850 nm, for the absorbance maximum of your sample. The SpectraMax® Plus 384 Microplate Reader from Molecular Devices can run both standard spectrophotometer and microplate reader applications on the same instrument. 0, 20 mmol L –1cal-cium chloride). Photometric Range: 0. 5. One plate carrier adapter is provided with the instrument. plates can be used in the SPECTRAmax PLUS 384. 5 mL of 1 M acetate buffer (pH 4. IMAP assays are based on binding of phosphate to immobilized metal coordination complexes on nanoparticles. Acceptor filter: 665 nm. TGFα release was calculated as described in the Results. Read up to 6 wavelengths in endpoint or kinetic mode for 96- and 384-well plates, and perform spectral scans to determine. Details. According to LDH assay, a 60 μL of reaction mixture containing catalyst (diaphorase/NAD+), dye solution of iodotetrazolium chloride and sodium lactate was. Bioz Stars score: 86/100, based on 1 PubMed citations. Versteegh, Yoran H. Following incubation for 30 min at room temperature, the reaction was quenched by addition of 100 μl stop solution (2 N H 2 SO 4) and absorbance was read using microplate reader (SpectraMax 340; Molecular Devices Co. 003 OD Stray light ≤ 0. CD154 detection in equine PBMC by an anti-human CD154 antibody (clone 5C8) was confirmed by Western blots and then applied for flow cytometry. chen@case. Column chromatography was performed on silica gel (Kieselgel 60, 70–230 mesh, Merck). Please refer to photos for further. The Gemini EM Reader with dual monochromators allows researchers to try new and novel dyes without having toThe absorbance was determined to be 450 nm using an ELISA reader (SpectraMax® 340, Molecular Device Co. The assays contained 0. TheSpectraMax®340PC384,SpectraMax®190,andVersaMax™microplate spectrophotometersproviderapidandsensitivemeasurementsofavarietyofanalytesacross. 0112-0115J 9 Chapter1:Introduction TheSpectraMax®M3Multi-ModeMicroplateReader,SpectraMax®M4Multi-ModeMicroplate Reader,SpectraMax®M5Multi-ModeMicroplateReader,andSpectraMax®M5eMulti-ModeOpening the Drawer Manually on page The SpectraMax ABS can accommodate standard 96-well plates. 0112-0126B 8 Chapter1:Introduction TheSpectraMax®340PC384,SpectraMax®190,andVersaMax™microplate. For FXII, at various times, 90 μL of reaction was mixed with SBTI (500nM) and S-2302 (0. chen@case. We selected the response at t=10 h for statistical analysis, because at that time point the response variation was largest. The OD 490 was measured with an absorbance microplate reader (SpectraMax 340; Molecular Devices). Immunofluorescence and Aggregation Analysis. The intensity of red color formed in the assay was measured at a wavelength of 490 nm with an ELISA reader (SpectraMax 340, Molecular Device Co. Products : SpectraMax Plus 384 reader, SpectraMax 190 reader, SpectraMax 340PC 384 (Obsolete), SpectraMax 250 (Obsolete), SpectraMax 340 (Obsolete), VersaMax reader How does stray light affect my OD reading? The basic problem caused by stray light (light you don't want, getting into the read) is a falsely low OD seen. The SpectraMax® ABS and ABS Plus absorbance microplate readers provide the flexibility, sensitivity, and convenience for a wide range of assays such as ELISAs, microbial growth, and protein quantitation. Assay setup for a 384-well low-volume plate. This Molecular Devices SPECTRAmax 340PC incorporates a holographic grating monochromator which allows you to specify a precise wavelength, from 340 nm to 850 nm, for the absorbance maximum of your sample. D.